Fig. 1. The overall cryo-EM structure of GHRH–GHRHR–G_s complex.

a Cryo-EM density map that illustrates the GHRH–GHRHR–G_s complex and the disc-shaped micelle. The unsharpened cryo-EM density map at the 0.005 threshold shown as light gray surface indicates a micelle diameter of 10 nm. The colored cryo-EM density map is shown at 0.028 threshold. b GHRH–GHRHR–G_s complex model and GHRHR ECD crystal structure model docked into the cryo-EM map. c Cartoon representation of the GHRH–GHRHR–G_s complex is shown with annular lipids in purple stick representation. Lime green, GHRHR; blue, GHRH; yellow, G_s Ras-like domain; red, Gβ; orange, Gγ; gray, Nb35; plum, lipid, and cholesterol.