Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Oct 15;10:17379. doi: 10.1038/s41598-020-74360-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The ER stress response is not activated in coa mutants during the OS growth period. (A) Quantitative PCR of 60-hpf wild-type and coa mutant heads and 48-hpf wild-type embryos treated with DMSO and tunicamycin, using primers for the xbp-1, chop1, and ef1α genes. PCR amplified band images for xbp-1, chop1, and ef1α genes are cropped from the original full-length electrophoretic gel images shown in Fig. S5. The alternative spliced form of xbp-1 and increased chop1 mRNA expression were observed only in tunicamycin-treated embryos. (B) The ratio of the spliced form relative to the non-spliced form of xbp-1 mRNA. We carried out three independent sets of PCR reactions for wild type, coa mutants, DMSO and Tunicamycin treatment. The same set of PCR reactions are connected with the line. The difference of coa mutant heads relative to wild-type heads, and Tunicamycin-relative to DMSO-treated embryos in xbp1 spliced/non-spliced mRNA ratio were evaluated by Ratio paired t-test, two-tailed. p** < 0.01. There was no significant difference between wild type and coa mutants. (C) Ratio of chop1 mRNA relative to ef1α mRNA. The difference of coa mutant heads relative to wild-type heads, and Tunicamycin-relative to DMSO-treated embryos in chop1 mRNA expression were evaluated by Ratio paired t-test, two-tailed. p* < 0.05. There was no significant difference between wild type and coa mutants.