Table 2.

Comparison between normal dose exposures versus optimized dose exposures using typical high-resolution microscope systems and parameters.

	Exposures/h with 1 exposure/hole (K2)	Exposures/h with 4 exposures/hole (K2)	Exposures/h with 1 exposure/hole (K3)	Exposures/h with 4 exposures/hole (K3)
Normal dose (~66 e-/Å2)	108	121	195	237
Optimized dose (~17 e-/Å2)	178	190	242	273
% increase in collection efficiency	65%	57%	25%	15%

Each entry reports the number of exposures collected using the normal versus denoised optimized doses using either a K2 or K3 camera on a Titan Krios and collecting only one exposure per hole versus four exposures per hole. The optimized dose yields more than a 50% increase in throughput on the K2 camera and still yields a modest improvement in throughput on the newer K3 camera.