Table 1.
Effect of eslicarbazepine acetate (300 μM) on Na+ current characteristics in MDA-MB-231 and HEK-Nav1.5 cells.1
| A. MDA-MB-231 cells | ||||
|---|---|---|---|---|
| Parameter | Control | ESL | P value | N |
| Vthres (mV) | -45.7 ± 1.7 | -45.0 ± 1.4 | 0.58 | 13 |
| Vpeak (mV) | 3.1 ± 2.1 | -3.9 ± 2.7 | 0.056 | 13 |
| Activation V½ (mV) | -19.3 ± 1.4 | -22.0 ± 1.5 | 0.095 | 12 |
| Activation k (mV) | 10.6 ± 0.7 | 9.3 ± 0.8 | 0.076 | 12 |
| Inactivation V½ (mV) | -80.6 ± 0.7 | -86.7 ± 1.2 | < 0.001 | 13 |
| Inactivation k (mV) | -4.8 ± 0.4 | -7.4 ± 1.7 | 0.139 | 13 |
| Peak current density at -10 mV (pA/pF) | -14.8 ± 3.9 | -8.0 ± 2.5 | < 0.001 | 13 |
| Persistent current density at -10 mV (pA/pF) | -0.15 ± 0.05 | -0.02 ± 0.07 | 0.13 | 12 |
| Tp at -10 mV (ms) | 2.1 ± 0.2 | 1.9 ± 0.2 | < 0.01 | 13 |
| τf at -10 mV (ms) | 1.3 ± 0.1 | 1.3 ± 0.2 | 0.954 | 13 |
| τs at -10 mV) (ms) | 10.0 ± 2.3 | 6.9 ± 2.0 | 0.289 | 13 |
| τr (ms) | 6.0 ± 0.5 | 8.7 ± 0.7 | < 0.05 | 10 |
| B. HEK-Nav1.5 cells | ||||
| Parameter | Control | ESL | P value | N |
| Vthres (mV) | -55.0 ± 1.7 | -54.0 ± 2.2 | 0.758 | 10 |
| Vpeak (mV) | -26.0 ± 2.2 | -24.0 ± 4.3 | 0.591 | 10 |
| Activation V½ (mV) | -39.4 ± 1.3 | -44.2 ± 1.8 | < 0.05 | 10 |
| Activation k (mV) | 5.3 ± 1.3 | 3.8 ± 0.7 | 0.361 | 10 |
| Inactivation V½ (mV) | -78.2 ± 2.5 | -88.3 ± 2.7 | < 0.001 | 10 |
| Inactivation k (mV) | -6.9 ± 0.4 | -9.8 ± 0.7 | < 0.001 | 10 |
| Peak current density at -10 mV (pA/pF) | -154.4 ± 24.0 | -33.1 ± 4.7 | < 0.001 | 12 |
| Persistent current density at -10 mV (pA/pF) | -0.61 ± 0.15 | -0.12 ± 0.05 | < 0.01 | 12 |
| Tp at -10 mV (ms) | 1.4 ± 0.2 | 1.9 ± 0.2 | < 0.001 | 14 |
| τf at -10 mV (ms) | 0.9 ± 0.1 | 1.2 ± 0.1 | < 0.001 | 12 |
| τs at -10 mV (ms) | 6.6 ± 0.8 | 20.8 ± 8.5 | 0.128 | 12 |
| τr (ms) | 4.5 ± 0.4 | 7.1 ± 0.6 | < 0.001 | 10 |
1ESL, eslicarbazepine acetate (300 µM); Vthres, threshold voltage for activation; Vpeak, voltage at which current was maximal; V½, half (in)activation voltage; k, slope factor for (in)activation; Tp, time to peak current; τf, fast time constant of inactivation; τs, slow time constant of inactivation; τr, time constant of recovery from inactivation. The holding potential was -120 mV. Results are mean ± SEM. Statistical comparisons were made with paired t-tests.