Table 2.
Effect of S-licarbazepine (300 μM) on Na+ current characteristics in MDA-MB-231 and HEK-Nav1.5 cells.1
| A. MDA-MB-231 cells | ||||
|---|---|---|---|---|
| Parameter | Control | S-Lic | P value | N |
| Vthres (mV) | -34.4 ± 2.0 | -35.7 ± 2.0 | 0.603 | 7 |
| Vpeak (mV) | 11.43 ± 4.4 | 10.0 ± 4.9 | 0.818 | 7 |
| Activation V½ (mV) | -12.9 ± 1.3 | -13.7 ± 1.4 | 0.371 | 7 |
| Activation k (mV) | 11.0 ± 0.5 | 11.9 ± 0.8 | 0.520 | 7 |
| Inactivation V½ (mV) | -71.8 ± 2.5 | -76.8 ± 2.2 | < 0.05 | 7 |
| Inactivation k (mV) | -6.8 ± 0.9 | -6.0 ± 1.2 | 0.302 | 7 |
| Peak current density at -10 mV (pA/pF) | -12.0 ± 3.1 | -6.9 ± 2.5 | < 0.001 | 9 |
| Persistent current density at -10 mV (pA/pF) | -1.3 ± 0.4 | -0.6 ± 0.2 | < 0.05 | 9 |
| Tp at -10 mV (ms) | 4.5 ± 0.4 | 5.1 ± 0.7 | 0.103 | 9 |
| τf at -10 mV (ms) | 3.8 ± 1.1 | 3.2 ± 0.4 | 0.553 | 7 |
| τs at -10 mV (ms) | 25.7 ± 7.0 | 27.1 ± 12.0 | 0.920 | 7 |
| τr (ms) | 6.8 ± 0.4 | 13.5 ± 1.0 | < 0.01 | 7 |
| B. HEK-Nav1.5 cells | ||||
| Parameter | Control | S-Lic | P value | N |
| Vthres (mV) | -50.0 ± 1.9 | -51.3 ± 3.5 | 0.598 | 9 |
| Vpeak (mV) | -18.0 ± 4.2 | -30.0 ± 5.6 | < 0.001 | 9 |
| Activation V½ (mV) | -32.8 ± 3.1 | -40.5 ± 3.4 | < 0.01 | 9 |
| Activation k (mV) | 5.9 ± 0.9 | 4.5 ± 1.1 | < 0.05 | 9 |
| Inactivation V½ (mV) | -75.9 ± 2.6 | -79.3 ± 4.1 | 0.116 | 9 |
| Inactivation k (mV) | -6.5 ± 0.4 | -8.1 ± 0.5 | < 0.05 | 9 |
| Peak current density at -10 mV (pA/pF) | -140.9 ± 26.8 | -77.2 ± 17.0 | < 0.001 | 13 |
| Persistent current density at -10 mV (pA/pF) | -0.9 ± 0.2 | -0.5 ± 0.2 | < 0.05 | 11 |
| Tp at -10 mV (ms) | 1.8 ± 0.5 | 2.3 ± 0.6 | < 0.01 | 13 |
| τf at -10 mV (ms) | 1.0 ± 0.04 | 1.3 ± 0.06 | < 0.001 | 11 |
| τs at -10 mV (ms) | 6.3 ± 0.5 | 7.3 ± 0.5 | < 0.05 | 11 |
| τr (ms) | 5.7 ± 0.7 | 8.0 ± 1.2 | < 0.01 | 10 |
1S-Lic, S-licarbazepine (300 µM); Vthres, threshold voltage for activation; Vpeak, voltage at which current was maximal; V½, half (in)activation voltage; k, slope factor for (in)activation; Tp, time to peak current; τf, fast time constant of inactivation; τs, slow time constant of inactivation; τr, time constant of recovery from inactivation. The holding potential was -120 mV. Results are mean ± SEM. Statistical comparisons were made with paired t-tests.