FIG 6.

Histopathological findings in the placenta and brain of a Brucella ceti-infected striped dolphin. (A) Placental villi with mononuclear and PMN inflammatory infiltrate (red arrowheads) and some detached placental epithelial cells (arrow). Hematoxylin and eosin (HE) stain. Magnification, ×40. (B) Immunoperoxidase labeling of intracellular Brucella antigens inside inflammatory PMNs and mononuclear cells (dark brown) invading the placental villi (example indicated by an arrow). Immunohistochemistry. Magnification, ×40. (C) Mononuclear infiltrate in infected brain meninges with the absence of PMNs. HE stain. Magnification, ×40. (D) Immunofluorescence of infected mononuclear cells (in green) in the cerebrospinal fluid of infected meninges. HE stain. Magnification, ×40. The expected outcomes of stranded striped dolphins with brucellosis are abortion, meningoencephalomyelitis, and death caused by the invading bacteria (172). (All photos courtesy of Rocio Gonzalez-Barrientos and Gabriela Hernández-Mora, reproduced with permission.)