Table 1. Differentially expressed genes upon exposure to sublethal dose of H2O2.
| ORF IDa | Gene | Function | COGsb | Log2FC | Adjusted p-value | FC (RT-qPCR)c |
|---|---|---|---|---|---|---|
| Up-regulated genes | ||||||
| LIMLP_02795 (LIC12927/LA0666) | ccp | Cytochrome C peroxidase | P | 4.764* | 5.31e-43 | 38.900 |
| LIMLP_05955 (LIC11219/LA2809) | ahpC | Peroxiredoxin/alkylperoxiredoxin reductase | O | 3.145* | 3.63e-20 | 11.742 |
| LIMLP_05960 (LIC11220/LA2808) | sufB | Fe-S cluster assembly protein | O | 1.056* | 1.60e-08 | 1.880 |
| LIMLP_10145 (LIC12032/LA1859) | katE | Catalase | P | 1.786 | 2.11e-08 | 3.477 |
| LIMLP_10150 (LIC12033/LA1858) | Ankyrin repeat-containing protein | S | 2.051* | 2.30e-11 | 4.183 | |
| LIMLP_10155 (LIC12034/LA1857) | perR | Regulator Fur familly | T | 2.319* | 1.02e-39 | 6.827 |
| LIMLP_17840 (LIC20008/LB010) | hemA | Glutamyl-tRNA reductase | H | 1.771* | 1.16e-10 | 3.389 |
| LIMLP_17845 (LIC20009/ LB011) | hemC/D | Porphobilinogen deaminase | H | 1.617* | 6.57e-13 | 2.328 |
| LIMLP_17850 (LIC20010/ LB012) | hemB | Delta-aminolevulinic acid dehydratase | H | 1.455* | 2.65e-14 | 2.064 |
| LIMLP_17855 (LIC20011/ LB013) | hemL | Glutamate-1 semialdehyde aminotransferase | H | 1.262* | 1.19e-07 | 2.193 |
| LIMLP_17860 (LIC20012/ LB014) | Signal transduction histidine kinase | T | 1.035* | 2.67e-03 | 2.470 | |
| LIMLP_17865 (LIC20013/ LB015) | Response regulator CheY | K | 1.166* | 1.01e-03 | 2.012 | |
| LIMLP_17870 (LIC20014/ LB016) | hemE | Uroporphyrinogen decarboxylase | H | 1.033* | 1.77e-02 | 2.059 |
| Down-regulated genes | ||||||
| LIMLP_18600 (LIC20149/ LB187) | Permease of the Major facilitator superfamily | P | -1.001 | 1.17e-04 | 0.894 |
Significantly up-and down-regulated genes upon a 30 min exposure to 10 μM H2O2 with a Log2FC cutoff of ± 1 and an adjusted p-value cutoff of 0.05.
a Gene numeration is according to Satou et al. [17]. Corresponding genes of L. interrogans serovar lai strain 56601 and serovar Copenhageni strain Fiocruz L1-130 are indicated in parenthesis.
b The COG functional categories are H, coenzyme transport and metabolism; K, transcription; O, posttranslational modification, protein turnover, chaperones; P, inorganic ion transport and metabolism; S, function unknown; T, signal transduction and metabolism.
c Fold change in gene expression upon a 30 min exposure to 10 μM H2O2 obtained by RT-qPCR experiments.
* Genes significantly up-and down-regulated by Volcano analysis (Log2FC cutoff of 1 and p-value cutoff of 0.05 as seen in Fig 1A).