Table 2. Differentially expressed genes upon perR inactivation.
| ORF IDa | Gene | Function | Log2FC | Adjusted p-value | FC (RT-qPCR)b |
|---|---|---|---|---|---|
| Down-regulated genes | |||||
| LIMLP_04090 (LIC12679/LA0980) | thic | Thiamine biosynthesis protein | -2.073 | 3.72e-02 | |
| LIMLP_04240 (LIC10889/LA3247) | tonb | Energy transducer TonB | -4.601 | 2.03e-13 | 0.00722 |
| LIMLP_04245 (LIC10890/LA3246) | exbD | Biopolymer transport protein ExbD/TolR | -4.606 | 7.90e-13 | 0.00737 |
| LIMLP_04250 (LIC10891/LA3245) | exbD | Biopolymer transport protein ExbD/TolR | -5.355 | 4.93e-15 | 0.00128 |
| LIMLP_04255 (LIC10892/LA3244) | exbB | Biopolymer transport protein ExbB/TolQ | -5.478 | 3.00e-22 | 0.00193 |
| LIMLP_04260 (LIC10893/LA3243) | Hypothetical | -1.519 | 4.27e-02 | 1.261 | |
| LIMLP_04270 (LIC10895-96/LA3242) | TonB-dependent receptor | -3.262 | 2.32e-05 | 0.0355 | |
| LIMLP_04275 (LIC10897/LA3241) | Hypothetical | -3.888 | 4.42e-05 | 0.00918 | |
| LIMLP_04280 (LIC10898/LA3240) | lipl48 | Hypothetical | -5.506 | 2.03e-13 | 0.00372 |
| LIMLP_09650 (LIC11935/LA1968)* | Hypothetical | -1.787 | 3.26e-02 | ||
| LIMLP_15470 (LIC10454/LA3793) | Putative hemolysin | -2.154 | 3.32e-12 | 0.3041 | |
| LIMLP_16720 (LIC13269/LA4102) | vicR | Response regulator | -1.611 | 5.80e-07 | 0.0752 |
| Up-regulated genes | |||||
| LIMLP_02010 (LIC13086/LA3867)** | Hypothetical lipoprotein | 1.029 | 4.08e-02 | ||
| LIMLP_02795 (LIC12927/LA0666)* | ccp | Cytochrome C peroxidase | 2.773 | 8.69e-18 | 7.943 |
| LIMLP_05955 (LIC11219/LA2809)* | ahpC | Peroxiredoxin/alkylperoxiredoxin reductase | 1.539 | 1.23e-05 | 2.01 |
| LIMLP_10145 (LIC12032/LA1859)* | katE | Catalase | 2.637 | 2.59e-24 | 4.897 |
| LIMLP_10150 (LIC12033/LA1858)* | Ankyrin repeat-containing protein | 2.867 | 4.65e-29 | 5.783 |
Significantly up-and down-regulated genes in the perR mutant with a Log2FC cutoff of ± 1 and an adjusted p-value cutoff of 0.05.
a Gene numeration is according to Satou et al. [17]. Corresponding genes of L. interrogans serovar lai strain 56601 and serovar Copenhageni strain Fiocruz L1-130 are indicated in parenthesis.
b Fold change in gene expression upon perR inactivation obtained by RT-qPCR experiments.
* ORFs significantly up-regulated upon exposure to H2O2 (Log2FC cutoff of -1, adjusted p-value cutoff of 0.05).
** ORFs significantly down-regulated upon exposure to H2O2 (Log2FC cutoff of -1, adjusted p-value cutoff of 0.05).