Figure 5.

Islet cell survival from dispersed human islets treated with VCS and TAC. (A, B) Cell death was assessed every 30 minutes over 48 hours using propidium iodide (PI) and Hoechst staining, with a significant increase seen in the presence of a β-cell toxic cytokine cocktail. (C) Shown is the maximum cell death normalized measurement (see methods) for each condition TAC and VCS dose, normalized to the DMSO control cultures. Results shown are quantified from 5 cultures per drug condition, over 2 separate runs.