Fig. 1.

a Effect of 7KC on endothelial cells in culture, determined using the trypan blue dye exclusion assay. a dose-dependent cytotoxicity of 7KC. hCMEC/D3 cells were treated with at 0–50 μM 7KC for 24 h. Cell viability is expressed as percentage viability from Vehicle Control. The oxysterol induced significant loss in viable cells at 30-100 μM. Data are represented as mean ± S.E.M. (n = 4). b protective effect of ET on 7KC cytotoxicity. hCMEC/D3 cells were pre-treated with different concentrations of ET for 1 h, followed by co-treatment with 7KC for 24 h. Co-treatment of cells with ET results in modulation of 7KC-induced loss of viable cells. Data are represented as mean ± S.E.M. (n = 4). * Significant difference compared to 7KC (p < 0.01)