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Effect of 7KC and ET on cellular localization of blood–brain barrier tight junction proteins, determined by immunocytochemistry. hCMEC/D3 cells were pre-treated with 1 mM ET for 1 h, followed by co-treatment with 50 μM 7KC for 24 h. 7KC induced a movement of ZO-1 and claudin-5 towards the nucleus, but this change of distribution was abolished by ET. Scale bar = 40 μm
