Fig. 7.

Effect of ET on 7KC-induced increase in mRNA expression of pro-inflammatory genes, determined by real-time RT-PCR. hCMEC/D3 cells were pre-treated with 1 mM ET for 1 h, followed by co-treatment with 30 μM 7KC for 24 h. Co-treatment of cells with ET results in modulation of 7KC-induced increase in IL-1β (a), IL-6 (b), IL-8 (c) and TNFα (d) mRNA expression in endothelial cells. Data are represented as mean ± S.E.M. ^ Significant difference compared to controls. * Significant difference compared to 7KC (both p < 0.01) (n = 6)