Fig. 8.

Effect of ET on 7KC-induced increase in mRNA expression of pro-inflammatory genes, determined by real-time RT-PCR. hCMEC/D3 cells were pre-treated with 1 mM ET for 1 h, followed by co-treatment with 50 μM 7KC for 24 h. Co-treatment of cells with ET results in modulation of 7KC-induced increase in NFkB and component genes (a), as well as COX-2 (b) mRNA expression in endothelial cells. This effect was abolished by concurrent treatment with an inhibitor of ET transporter, VHCl. Data are represented as mean ± S.E.M. (n = 4). ^ Significant difference compared to controls. * Significant difference compared to 7KC. ^# Significant difference compared to ET + 7KC (all p < 0.01)