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Construction of an isogenic htpsA mutant of S. suis 05ZYH33. (a) Schematic diagram of the construction process of the ΔhtpsA strain. (b) Combined PCRs of the ΔhtpsA mutant. (c) Reverse-transcription PCR analysis of htpsA gene transcripts. The primer pairs and templates used in the PCR analysis are indicated above the lanes. WT and ΔhtpsA represent genome DNA of the wild-type strain 05ZYH33 and mutant strain, respectively.
