Table 1.
Bacterial strains and plasmids used in this study.
| Strains/plasmids | Characteristics and/or function | Source |
|---|---|---|
| Strains | ||
| S. suis 05ZYH33 | Virulent strain isolated from a patient with STSS | Lab collection |
| S. suis Δcps2B | Isogenic Δcps2B deletion mutant of strain 05ZYH33; SpcR | 44 |
| S. suis ΔhtpsC | Isogenic ΔhtpsC deletion mutant of strain 05ZYH33; SpcR | 39 |
| S. suis ΔhtpsA | Isogenic ΔhtpsA deletion mutant of strain 05ZYH33; SpcR | This study |
| E. coli DH5α | Cloning host recombinant plasmids | Transgen |
| Plasmids | ||
| pMD18 – T | Cloning vector; AmpR | Takara |
| pUC19 | E. coli cloning vector, lacZ, AmpR | Takara |
| pUC::htpsA | A recombinant vector with the background of pUC19, designed for knock-out of htpsA; AmpR, SpcR | This study |
| pSET2 | E. coli-S. suis shuttle vector; SpcR | 35 |
The AmpR and SpcR represent ampicillin resistant gene and spectinomycin resistant gene respectively.