Figure 6.
Median lethal dose comparison for B. mallei WT and recombinant strains (repeat experiment). BALB/c mice were inoculated with a Microsprayer device to aerosolize the indicated numbers of bacterial CFU directly into the lungs (n = 5 mice/dose). The animals were then monitored daily for clinical signs of illness and morbidity. (a) Survival data and calculated LD50 values. (b–d) Tissues were collected from mice that survived challenge (day 27), homogenized, diluted, and spread on agar plates to determine bacterial loads. The symbols represent data from individual animals; horizontal lines represent the mean total number of CFU for each group.