Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 27;16(12):1785–1793. doi: 10.1080/15476286.2019.1658508

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Informa UK Limited, trading as Taylor & Francis Group

PMC Copyright notice

Figure 5. — CCND1 is a target of YTHDF2 which could influence mRNA stability.

(a) RIP analysis of the interaction of CCND1 with FLAG in 3T3-L1 cells transfected with control or YTHDF2-FLAG. Enrichment of CCND1 with FLAG was measured by qPCR and normalized to input. (b) Western blot analysis of FLAG and YTHDF2 in cells transfected with control and YTHDF2-FLAG plasmid. The right panel shows the relative protein levels quantified by densitometry and normalized to GAPDH. (c) Lifespans of CCND1 expression in cells transfected with the YTHDF2 overexpression plasmid compared with those of cells transfected with empty vector. Relative mRNA levels quantified by qPCR. (d) Western blot analysis of CCND1 in control, ZFP217 knockdown or YTHDF2 knockdown cells after MDI-induced for 24 h. The right panel shows the relative protein levels quantified by densitometry and normalized to GAPDH. (e) ORO staining of control, ZFP217 knockdown and ZFP217+ YTHDF2 knockdown cells after MDI-induced for 8 days. The data are presented as the mean ± SD of triplicate tests. *P< 0.05, **P< 0.01, ***P< 0.001 compared to the control group.