Figure 4.

AS27a and AS32a are abundant in cancer cell lines and their levels are elevated in some breast cancer patients.

(A) Percentage of Drosha splice variants AS27a and AS32a in cancer cell lines. The RNA-seq data of cancer cell lines were obtained from CCLE database. The cell lines with a minimum of 20 AS27a-specific reads or 40 AS32a-specific reads were sorted by the percentage of AS27a or AS32a and plotted in the left Y axis. Drosha expression level (log2-transformed FPKM values) in each corresponding cell line was plotted in the right Y axis. (B) Percentage of Drosha splice variants AS27a and AS32a in breast normal and tumour tissues. The RNA-seq data of normal and tumour tissues were obtained from TCGA database. Normal, n = 104; Tumour, n = 1162.