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Multiplex PCR detection of mcr genes. Purified plasmid pUC57 carrying each mcr gene was used as templates. Agarose gel electrophoresis (2.0%) was used to separate multiplex PCR products. M indicates the molecular size marker (Trans100bp plus DNA ladder, TransGen Biotech, China). The size of each amplicon is indicated on the right side. NC, empty vector pUC57 was included as negative control.
