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Molecular detection of mcr genes in bacterial isolates by the multiplex PCR method. Colistin-resistant bacterial strains (43 isolates of E. coli and 17 isolates of K. pneumoniae) were tested for the presence of mcr genes. Total bacterial DNA was obtained by the boiling method and applied to the multiplex PCR assay. E. coli strain Top10 harboring the mcr-encoding plasmid was included as a positive control. NC, E. coli TOP10 with empty vector pUC57. M shows the molecular size marker.
