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. 2020 Oct 12;12:9999–10010. doi: 10.2147/CMAR.S261979

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© 2020 Wang et al.

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The prediction and confirmation of the interaction between miR-1271-5p and E2F5. (A) The binding sites of miR-1271-5p and E2F5. (B and C) The luciferase activity was determined (n=3). (D and E) The mRNA and protein level of E2F5 were detected by qRT-PCR and Western blot in SKOV3/DDP and OVCAR3/DDP cells transfected with miR-NC or miR-1271-5p (n=3). (F–I) The mRNA and protein level of E2F5 were detected in DDP-resistant and DDP-responsive OC tissues (F and G) and cells (H and I). (J) The association between miR-1271-5p and E2F5 expression was detected (n=3). *P<0.05.