Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Nicotiana benthamiana) | NbAGO4-1 | GenBank | DQ321490 | |
| Gene (Nicotiana benthamiana) | NbAGO4-2 | GenBank | DQ321491 | |
| Gene (Solanum lycopersicum) | SlAGO4a | Sol Genomics | SOLYC01G008960 | |
| Gene (Solanum lycopersicum) | SlAGO4b | Sol Genomics | SOLYC06G073540 | |
| Gene (Solanum lycopersicum) | SlAGO4d | Sol Genomics | SOLYC01G096750 | |
| Antibody | anti-V2 (Rabbit polyclonal) | This work | N/A | WB (1:2000) |
| Antibody | anti-Actin (Rabbit polyclonal) | Agrisera | Cat# AS13 2640 |
WB (1:5000) |
| Antibody | anti-FLAG (Mouse monoclonal) | Sigma | Cat# F3165 | IP: (5 µg per gram of tissue) WB (1:5000) |
| Antibody | anti-HA (12CA5) (Mouse monoclonal) | Roche | Cat# 11583816001 | WB (1:5000) |
| Antibody | anti-GFP (Mouse monoclonal) |
Abiocode | Cat# M0802-3a | WB (1:5000) |
| Commercial assay or kit | ClonExpress MultiS One Step Cloning Kit | Vazyme | Cat# C113-01 | |
| Commercial assay or kit | pENTR/D-TOPO Cloning Kit | Invitrogen | Cat# K240020SP | |
| Commercial assay or kit | Gateway LR Clonase II Enzyme Mix | Invitrogen | Cat# 11791100 | |
| Commercial assay or kit | QuickChange Lightning Site-Directed Mutagenesis Kit | Agilent Technologies | Cat# 210518 | |
| Commercial assay or kit | Plant RNA kit | OMEGA Bio-tek | Cat# R6827 | |
| Commercial assay or kit | iScriptTM cDNA Synthesis Kit | Bio-Rad | Cat# 1708890 | |
| Commercial assay or kit | DNeasy Plant Mini Kit | QIAGEN | Cat# 69104 | |
| Commercial assay or kit | EpiTect Plus DNA Bisulfite Kit | QIAGEN | Cat# 59124 | |
| Commercial assay or kit | QIAquick PCR Purification Kit | QIAGEN | Cat# 28106 | |
| Commercial assay or kit | Superscript III first-strand synthesis system | Thermo Fisher Scientific | Cat# 18080051 | |
| Software, algorithm | Cytosine methylation analysis Kismeth | Gruntman et al., 2008 | PMID:18786255 | http://katahdin.mssm.edu/kismeth/revpage.pl |