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. 2020 Sep 21;9:e55589. doi: 10.7554/eLife.55589

Figure 5. Apelin signaling positively regulates EC metabolism.

(A - B) Extracellular acidification aate (ECAR) (A) and oxygen consumption rates (OCR) (B) in siScr and APLN+APLNR siRNA-treated HUVECs under basal conditions and in response to oligomycin, fluoro-carbonyl cyanide phenylhydrazone (FCCP) and antimycin A (AA)/rotenone. (A) Reduced basal and maximal glycolytic activity in APLN+APLNR siRNA-treated compared to siScr-treated HUVECs. (B) No significant difference in oxygen consumption in APLN+APLNR siRNA-treated compared to siScr-treated HUVECs. (C) Reduced c-MYC levels in APLN+APLNR siRNA-treated compared to siScr-treated HUVECs. (D) RT-qPCR analysis of PFKFB3 mRNA levels in APLN+APLNR siRNA-treated compared to siScr-treated HUVECs. (E) Confocal projection images of the blood vasculature in the trunk region of a 54 hpf Tg(kdrl:HsHRAS-mCherry) animal injected with an EGFP:fli1a:pfkfb3 plasmid. Arrowheads point to formed DLAV fragments while asterisks indicate missing DLAV fragments. (F) Quantification of the rescue of the DLAV fragment by mosaic pfkfb3 overexpression in aplnrb -/- embryos. n.s. not significant (two-tailed t-test). Scale bar: E, 50 µm.

Figure 5.

Figure 5—figure supplement 1. Schematic model.

Figure 5—figure supplement 1.

Schematic model of the developing blood vessels in the zebrafish trunk depicting the expression of Apelin and Aplnr and the relationship between Notch signaling and Apln expression during ISV sprouting.