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. 2020 Sep 21;9:e55589. doi: 10.7554/eLife.55589

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
genetic reagent (D. rerio) Tg(fli1a:EGFP)y1 Lawson and Weinstein, 2002 ZFIN: y1
genetic reagent (D. rerio) Tg(fli1a:nEGFP)y7 Roman et al., 2002, ZFIN: y7
genetic reagent (D. rerio) Tg(kdrl:HsHRAS-mCherry)s896 Chi et al., 2008 ZFIN: s896
genetic reagent (D. rerio) aplnramu296 Helker et al., 2015 ZFIN: mu296
genetic reagent (D. rerio) aplnrbmu281 codes for another allele of aplnrb from Helker et al., 2015 ZFIN: mu281
genetic reagent (D. rerio) aplnmu267 Helker et al., 2015 ZFIN: mu267
genetic reagent
(D. rerio)
Tg(hsp70:bmp2b)fr13 Chocron et al., 2007 ZFIN: fr13
genetic reagent (D. rerio) apelabr13 Chng et al., 2013, ZFIN: br13
genetic reagent (D. rerio) Tg(fli:lifeact-GFP)mu240 Hamm et al., 2016 ZFIN: mu240
genetic reagent (D. rerio) Tg(fli1a:Hsa.B4GALT1-mCherry)bns9 Kwon et al., 2016 ZFIN: bns9
genetic reagent (D. rerio) Tg(hsp70:apln)mu269 This manuscript ZFIN: mu269
genetic reagent (D. rerio) Tg(kdrl:HsHRAS-EGFP)mu280 This manuscript ZFIN: mu280
genetic reagent (D. rerio) Tg(apln:EGFP)bns157 This manuscript ZFIN: bns157
genetic reagent (D. rerio) Tg(aplnrb:aplnrb-TagRFP-
sfGFP)bns309
This manuscript ZFIN: bns309
antibody anti-FOXO1 (rabbit monoclonal) Cell Signaling Technology Cat#2880 (1:1000)
antibody anti-pThr24FOXO1/pThr32FOXO3a (rabbit monoclonal) Cell Signaling Technology Cat#9464 (1:1000)
antibody anti-c-MYC (rabbit polyclonal) Cell Signaling Technology Cat#9402 (1:1000)
antibody anti-Tubulin (rabbit polyclonal) Cell Signaling Technology Cat#2148 (1:1000)
other Taqman probe APLN Thermo Fisher Scientific Hs00175572_m1
other Taqman probe APLNR Thermo Fisher Scientific Hs00270873_s1
other Taqman probe PFKFB3 Thermo Fisher Scientific Hs00270873_s1
other Taqman probe ACTB Thermo Fisher Scientific Hs01060665_g1
commercial assay or kit In-Fusion HD Cloning Plus Takara Bio Cat# 638910
transfected construct (human) APLN Dharmacon Cat# L-017023-01-0005 50 nM
transfected construct (human) APLNR Dharmacon Cat# L-005430-00-0005 50 nM
transfected construct (human) ON-TARGETplus Non-targeting Pool Dharmacon Cat# D-001810-10-05 50 nM
commercial assay or kit mMessage mMachine SP6 Transcription Kit Thermo Fisher Scientific Cat# AM1340
commercial assay or kit DIG RNA labelling kit Roche Cat# 11277073910
commercial assay or kit SuperScript III First-Strand Synthesis System Thermo Fisher Scientific Cat#18080051
commercial assay or kit RNA Clean and Concentrator Kit Zymo Research Cat# R1013
software, algorithm ZEN Blue 2012 Zeiss, Germany
software, algorithm ZEN Black 2012 Zeiss, Germany
software, algorithm Imaris - Version 8.4.0 Bitplane, UK
software, algorithm GraphPad Prism 6 GraphPad Software, USA