Table 2.
Quality Controls and Internal Standards
| Name | Used in | Monoisotopic mass (g/mol) | MRM method | Cone Voltage (V) | Collision energy (V) |
|---|---|---|---|---|---|
| Caffeine | logD7.4 | 194.1 | (+) 195.1–138.0 | 35 | 16 |
| Cyclobenzaprine | logD7.4 | 275.2 | (+) 276.0–216.0 | 35 | 22 |
| Nicardipine | logD7.4 | 479.2 | (+) 480.2–315.0 | 35 | 22 |
| Verapamil | logD7.4, Sol, Mics, Heps | 454.3 | (+) 455.3–165.0 | 70 | 28 |
| Astemizole | Sol | 458.2 | (+) 459.3–135.1 | 35 | 40 |
| Phenytoin | Sol | 252.1 | (+) 253.1–182.1 | 35 | 16 |
| Chlorpromazine | Sol | 318.1 | (+) 319.1–86.0 | 35 | 22 |
| Warfarin | PPB | 308.1 | (+) 309.1–162.9 | 5 | 16 |
| Propranolol | PPB | 259.2 | (+) 260.1–116.0 | 33 | 16 |
| Metoprolol | PPB, Mics | 267.2 | (+) 268.2–116.3 | 35 | 16 |
| 5,5-diethyl-1,3-diphenyl-2-iminobarbituric acid | PPB, Mics, Heps | 335.2 | (+) 336.2–195.0 | 46 | 28 |
| Diclofenac | Mics | 295.0 | (+) 296.0–214.2 | 35 | 40 |
| Imipramine | Mics | 280.2 | (+) 281.4–86.1 | 35 | 16 |
| Phenacetine | Mics | 179.1 | (+) 180.1–110.2 | 35 | 22 |
| Benzydamine | Mics | 309.2 | (+) 310.2–86.1 | 35 | 16 |
| Bosentan | Heps | 551.2 | (+) 552.2–202.0 | 35 | 28 |
| Dofetilide | Heps | 441.1 | (+) 442.1–197.9 | 5 | 28 |
| Indapamide | Heps | 365.1 | (+) 366.1–132.0 | 22 | 16 |
| Lorazepam | Heps | 320.0 | (+) 321.0–274.9 | 35 | 22 |
| Terfenadine | Heps | 471.3 | (+) 472.3–436.2 | 35 | 28 |
Heps, assay for metabolic stability in rat hepatocytes; Mics, assay for metabolic stability in human liver microsomes; PPB, plasma protein binding; Sol, solubility.