. 2020 Sep 19;104(21):9205–9218. doi: 10.1007/s00253-020-10878-4

Table 3.

Kinetic constants of PeAAO2 compared with those of other AAOs

		PeAAO2 from P. eryngii expressed in P. pastoris^a	PeAAO from P. eryngii expressed in A. nidulans^b	PeAAO FX9 variant from P. eryngii expressed in P. pastoris^c
p-Anisyl alcohol	K_M (μM)	24.3 ± 0.8	27	37
	k_cat (s⁻¹)	59.2 ± 0.04	142	70
	k_cat/K_M (mM⁻¹ s⁻¹)	2436	5230	1909
Benzyl alcohol	K_M (μM)	599.6 ± 18.7	632	440
	k_cat (s⁻¹)	12.8 ± 0.01	30	34
	k_cat/K_M (mM⁻¹ s⁻¹)	21.39	47	78
Cinnamyl alcohol	K_M (μM)	2740 ± 103	n.d.	n.d.
	k_cat (s⁻¹)	125.5 ± 0.1	n.d.	n.d.
	k_cat/K_M (mM⁻¹ s⁻¹)	45.80	n.d.	n.d.
Cumic alcohol	K_M (μM)	1912 ± 42.4	n.d.	n.d.
	k_cat (s⁻¹)	160.8 ± 0.1	n.d.	n.d.
	k_cat/K_M (mM⁻¹ s⁻¹)	84.1	n.d.	n.d.
trans,trans-2,4-hexadienol	K_M (μM)	143.6 ± 11.5	94	106
	k_cat (s⁻¹)	68.8 ± 0.05	119	89
	k_cat/K_M (mM⁻¹ s⁻¹)	479.3	1270	840
Piperonyl alcohol	K_M (μM)	59.1 ± 3.0	n.d.	n.d.
	k_cat (s⁻¹)	35.5 ± 0.02	n.d.	n.d.
	k_cat/K_M (mM⁻¹ s⁻¹)	600.2	n.d.	n.d.
Veratryl alcohol	K_M (μM)	446.6 ± 7.5	540	410
	k_cat (s⁻¹)	47.2 ± 0.03	114	57
	k_cat/K_M (mM⁻¹ s⁻¹)	105.7	210	139

n.d. not determined

^a(This study), 100 mM sodium phosphate buffer pH 6.0. 25 °C, all measurements in triplicate

^b(Ferreira et al. 2006), 100 mM sodium phosphate buffer pH 6.0, 24 °C

^c(Viña-Gonzalez et al. 2018), 100 mM sodium phosphate buffer pH 6.0, 25 °C