Figure 4.

Protein Kinase R Is Downregulated by Lytic KSHV Infection

(A) Flow cytometry analysis of HuAR2T.rKSHV.219 cells with latent (top panel) and lytic (bottom panel) KSHV infection.

(B) Immunoblot analysis of uninfected HuAR2T cells (lane 1) and HuAR2T.rKSHV.219 cells (lanes 2–5) in latent and lytic stages of KSHV infection.

(C) Flow cytometry analysis of HuAR2T.rKSHV.219 cells with stable expression of PKR-4xHA and GFP-HA untreated or treated with reactivation mix and stained with anti-HA tag antibody. The signal from the HA tag is compared between the RFP+ population (red line) and the latent cells (black line).

(D) Immunoblot analysis of HuAR2T.rKSHV.219 cells transduced with LV RTA or control LV, sorted on RFP+, and probed with the indicated antibody.

(E) qRT-PCR analysis of the host and viral gene expression in cells with the lytic versus latent stage of infection. Data are represented as mean ± SEM.

(F) Immunoblot analysis of HuAR2T.rKSHV.219 Cas9 cells treated with RTA reactivation mix and poly(I:C) and probed with the indicated antibody.