Kinetic Profiling of KSHV Proteins
(A) Schematic overview of the proteomic analysis of cells with latent versus lytic infection at 36, 48, and 60 h upon KSHV reactivation.
(B) Hierarchical cluster analysis of all viral proteins quantified. The heatmap diagram shows temporal and PAA-sensitivity profiles for 62 KSHV ORFs. Rows and columns represent individual viral ORFs and experimental samples, respectively.
(C–G) Average temporal and PAA-sensitivity profiles of viral proteins in clusters 1–5. Protein abundance is calculated as a fraction of maximum TMT reporter ion intensity.
See also Figures S6 and S7.