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. 2020 Oct 16;11(10):870. doi: 10.1038/s41419-020-03087-4

Fig. 1. Enolase 1 (ENO1) is upregulated in stem cell-like cells enriched from PAMC-82 and SNU16 cells by spheroid-forming culture.

Fig. 1

A Analysis of the self-renewal abilities of PAMC-82, SNU16 parental cells, and spheroids using methylcellulose spheroid-formation assay. Scale bar, 100 μm. B Tumorigenicity assay in PAMC-82, SNU16 parental cells, and spheroids. C Western blot analysis for the expression of ENO1 in PAMC-82, SNU16 parental cells, and spheroids. spheroids: GSCSs enriched from parental cells cultured in spheroid-formation conditions and passaged to the third-passage. Results are from representative experiments in triplicate and shown as the mean ± standard deviation (SD). *P < 0.05, **P < 0.01, ***P < 0.001.