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. 2020 Oct 16;11:5246. doi: 10.1038/s41467-020-18681-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Demonstration of UV irradiation of the array surface with 100 µm circular spots arranged in a (3 ⋅ 4) patterned format on 1.2 mm² of surface area. UV irradiation is not limited to this particular patterning and may be pixel-wise (1920 × 1080) changed on-demand with our photolithographic system. Any spot on the surface is individually addressable in terms of spatial location and the total amount of UV irradiation time. b Visualization of G homopolymeric oligonucleotide synthesis post system optimization via the splint-end ligation of a probe sequence containing a 3^′-Cy3 fluorophore using the (3 ⋅ 4) pattern. c Results of base transition normalization in which the total illumination time was adjusted for any base transition that may be encountered during multiplexed synthesis. The left axis indicates the composition of the last 4 bases on the 3^′-terminus of surface initiator oligonucleotide and the top axis indicates the nucleotide that was incorporated onto the respective initiator oligonucleotide. The optimal illumination time is indicated in each base transition box and was determined by splint-end ligated 3^′-Cy3 fluorescent signal. d Box plots indicating NGS analysis of base transition normalization. Graphs show the normalized nucleotide (nt) extension length distribution for all possible base transitions with red pluses being statistical outliers. Source data are provided as a Source Data file.