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. 2020 Oct 16;11:5261. doi: 10.1038/s41467-020-18950-3

Fig. 4. Cluster N2 is reduced in the piericidin-bound enzyme but its surrounding structure does not change.

Fig. 4

a EPR spectra showing reduced clusters in the piericidin-treated sample as prepared (black) and following addition of 10 mM NADH (grey). b EPR spectra showing reduced clusters in the piericidin-free control samples: the turnover control as prepared (black) and following addition of 10 mM NADH (grey) and the nonturnover control as prepared (red). EPR conditions: 9.5 GHz (X-band), temperature 20 K, microwave power 2.0 mW, modulation frequency 100 kHz, modulation amplitude 0.7 mT. c Key residues around N2 in the piericidin-bound (teal) and active (wheat) states. Distances are in Å. NDUFS2-Tyr108, the ligand to the piericidin headgroup, is shown for reference.