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. 2020 Oct 16;11:5261. doi: 10.1038/s41467-020-18950-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a One-site competitive inhibition mechanism. b Two-site competitive inhibition mechanism. In both cases, rapid reoxidation of ubiquinol by AOX (k₄) prevents appreciable levels accumulating. Experimentally-measured rates (average ± standard deviation, n = 3) are shown in K_M and IC₅₀ plots, alongside the best-fit predictions from the models (see Supplementary Table 3 for parameters). c Plots of trends in K_M,app, V_max,app, IC₅₀ and Hill slope produced by fitting K_M and IC₅₀ curves to the experimental data (black points, error bars are 95% confidence intervals) and to the output data from the models. The SSR values are 201 and 46.7 for a and b, respectively.