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. 2020 Oct 15;10:17393. doi: 10.1038/s41598-020-74534-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in transcript levels upon the downshift in salt concentration in WT and ΔlexA strains. Total RNA was isolated from cells before (− 1 h) and after 1 h of incubation with 500 mM NaCl (0 h) and at time points indicated after transferred to the normal BG-11 medium (0.5, 1 and 3 h). 5 μg of total RNA was applied to each lane for RNA gel blot analysis and transcripts were detected by using single-stranded riboprobes of ggpS, ggpP, hspA and nblB. rRNA was visualized with methylene blue staining to show the equal RNA loading. The location of 23S and 16S rRNA was shown by asterisks.