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. 2020 Oct 16;11:5258. doi: 10.1038/s41467-020-18991-8

Fig. 6. Compared to Δmyr1 parasites, Δgra45 parasites are more susceptible to IFNγ and less virulent in mice.

Fig. 6

a Toxoplasma genes that have at least four sgRNAs present after the 3rd passage in naïve BMDM in the three independent screens are rank-ordered according to their IFNγ vs. Naïve BMDM fitness scores. Data are displayed as average fitness scores (black plots) ± SEM (gray lines). b Mean IFNγ vs. Naïve BMDM fitness of GRA45, MYR1, MYR2, MYR3, and MYR4. Data are displayed as mean ± SEM with independent screens (n = 3) indicated by the same color dots (S1: white; S2: blue; S3: red). The significant difference was analyzed with one-way ANOVA with Tukey’s multiple comparisons test. c Murine BMDMs prestimulated with or without IFNγ (1, 5, 10, or 50 ng/mL) were infected with luciferase-expressing WT, Δmyr1, Δgra45, or Δgra45 + GRA45HA parasites (MOI of 0.25) for 24 h. Parasite growth for each strain was measured by luciferase assay and the growth in IFNγ-activated BMDMs is expressed relative to growth in naïve BMDMs. Data are displayed as connecting lines with mean of four independent experiments ± SEM. The significant difference was analyzed with two-way ANOVA with Tukey’s multiple comparisons test. d CD-1 mice were i.p. infected with 100 tachyzoites of WT, Δmyr1, Δgra45, or Δgra45 + GRA45HA parasites and survival of mice was monitored for 30 days. Data are displayed as Kaplan–Meier survival curves (n = 9 mice infected with WT or Δgra45 parasites, n = 5 mice infected with Δmyr1 parasites, and n = 4 mice infected with Δgra45 + GRA45HA parasites). The significant difference was analyzed with Log-rank (Mantel–Cox) test.