Table 2.

The Sedimentation Coefficient and Calculated Molecular Weight of PDZ3 Variants in AUC Measurements at 1 mg/mL, pH 7.5, and 25°C

Name	Weighted Average Sedimentation Coefficient (S)	Calculated molecular weight (kDa)
PDZ3-wt	1.52	10,590.1
PDZ3-F340A	1.51	10,459.3
PDZ3-L342A	1.49	10,490.9
PDZ3-I389A	1.48	10,495.6
PDZ3-L349A	1.53	9656.1

Sedimentation velocity analysis of PDZ3 variants was performed at 25°C, and experimental data were analyzed using SEDFIT (32) and SEDNTERP (33).