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Tm and van’t Hoff Enthalpy (ΔHvan’t Hoff (Tm)) of PDZ3 Variants by Two-State Analysis in CD Measurement at 0.5 mg/mL, pH 7.5, and 1°C/min Scan Rate. These Thermodynamic Parameters Were Calculated by Fitting Thermal Denaturation Curves of PDZ3 Variants
| Name | Tm (°C) | ΔHvan’t hoff (Tm) (kJ/mol) |
|---|---|---|
| PDZ3-wt | 72.58 | 249.13 |
| PDZ3-F340A | 72.98 | 350.08 |
| PDZ3-L342A | 70.97 | 339.13 |
| PDZ3-I389A | 60.99 | 237.23 |
| PDZ3-L349A | 69.74 | 241.75 |
