Figure 1.

Resolution and map deposition trends in single-particle cryo-EM. Shown are the annual (blue dotted curve) and cumulative (orange curve) map depositions into the Electron Microscopy Data Bank (EMDB). The median resolution of deposited maps per year is also shown (gray curve); future cryo-EM methodological and hardware developments, such as those discussed in this review, will likely improve the median resolution to 4 Å or better over the next decade. To see this figure in color, go online.