Table 1.

Summary of Fluorescence Properties of Various Green Fluorescence Proteins

Protein	Average Fluorescence Lifetime (ns)	Absmax (nm)	Emmax (nm)	Molecular Brightness (a.u.)	Fluorescence Stability (a.u.)	Maturation (min)
Autofluorescence	2.40 ± 0.20
slmGFP	1.80 ± 0.15	501	513	0.65 ± 0.07	0.97 ± 0.13	4a (59)
myeGFP (22)	2.00 ± 0.18	501	513	0.76 ± 0.41	0.94 ± 0.19	n.d.
sfGFP (60)	2.50 ± 0.14	487	511	1.00 ± 0.18	0.90 ± 0.07	6 (61)
sfGFP_cp8 (62)	2.52 ± 0.20	487	510	n.d.	0.77 ± 0.14	n.d.
sfGFP_cp7 (62)	2.72 ± 0.11	486	510	n.d.	0.87 ± 0.06	n.d.
sfGFP_cp3 (62)	2.78 ± 0.04	487	510	n.d.	0.84 ± 0.10	n.d.
no-sfsfGFP (62)	2.84 ± 0.06	489	511	n.d.	0.90 ± 0.08	n.d.
mNeonGreen (63)	2.90 ± 0.12	506	517	1.71 (63)	0.80 ± 0.27	<10 (63)
Clover (F64L) (62)	3.00 ± 0.03	506	517	n.d.	0.67 ± 0.12	n.d.
Clover (64)	3.00 ± 0.09	505	517	1.56 (63)	0.69 ± 0.37	30 (62)
sfGFP (L64F) (62)	3.10 ± 0.01	487	511		0.78 ± 0.09	n.d.
Envy (65)	3.23 ± 0.02	486	509	1.06 ± 0.21	0.92 ± 0.12	n.d.
GFPγ (66)	3.33 ± 0.08	493	509	1.71 ± 0.35	0.78 ± 0.25	n.d.
Ivy (65)	3.44 ± 0.06	500	515	0.98 ± 0.25	0.84 ± 0.12	n.d.
NowGFP (10 s) (53)	3.90 ± 0.09	492	502	0.79 ± 0.14	0.33 ± 0.13	n.d.

Proteins that were selected for further studies are indicated in bold. Average fluorescence lifetime corresponds the intensity weighted fluorescence lifetime. Fluorescence stability is defined as the percentage of fluorescence remaining after 20 s of illumination (corrected for the autofluorescence). Molecular brightness (as defined in the text) and fluorescence stability values correspond to the mean of five measurements per strain. Errors are expressed by standard deviations. Abs, absorption; a.u., arbitrary unit; Em, emission; n.d., not determined.

^aIn contrast to the other maturation times that integrate protein folding and fluorophore maturation together, this value here only relates to fluorophore oxidation.