Skip to main content
. 2020 Oct 7;2020:6801587. doi: 10.1155/2020/6801587

Figure 3.

Figure 3

Immunofluorescence staining of CSF1R and CSF1 with NeuN in the brain at 48 h after HI. (a) Representative microphotographs of CSF1R or CSF1 with NeuN staining in the ipsilateral cortex of the sham, HI+vehicle, and HI+rh-CSF1 groups. (b) Quantitative analysis of CSF1R-positive NeuN cells in the ipsilateral cortex at 48 h after HI. (c) Quantitative analysis of CSF1-positive NeuN cells in the ipsilateral cortex at 48 h after HI. Green indicated CSF1R- or CSF1-positive staining, red indicated NeuN-positive neuron staining, and blue indicated DAPI-positive nuclear staining. Merge showed the colocalization of CSF1R or CSF1 with neurons. ∗P < 0.05 vs sham; #P < 0.05 vs vehicle; scale bar = 50 μm; n = 4 per group. The upper right panel of the brain slice indicates the location for staining analysis (small blue box).