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. 2020 Oct 7;2020:6801587. doi: 10.1155/2020/6801587

Figure 4.

Figure 4

Immunofluorescence staining of CSF1R or CSF1 with GFAP in the brain at 48 h after HI. (a) Representative microphotographs of CSF1R and CSF1 with GFAP staining in the ipsilateral cortex of the sham, HI+vehicle, and HI+rh-CSF1 groups. (b) Quantitative analysis (T test) of CSF1R-positive GFAP cells in the ipsilateral cortex at 48 h after HI. (c) Quantitative analysis of CSF1-positive GFAP cells in the ipsilateral cortex at 48 h after HI. Green indicated CSF1R- or CSF1-positive staining, red indicated GFAP-positive astrocytes staining, and blue indicated DAPI-positive nuclear staining. Merge showed the colocalization of CSF1R or CSF1 with astrocytes. ∗P < 0.05 vs sham; #P < 0.05 vs vehicle; scale bar = 50 μm; n = 4 per group. The upper right panel of brain slice indicates the location for staining analysis (small blue box).