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Effects of rh-CSF1 and PLCG2 inhibitor on downstream protein expression levels in the proposed signaling pathway with rh-CSF1 treatment at 48 h after HI. (a) Representative western blot bands of p-CSF1R, CSF1R, CSF1, p-PLCG2, p-PKA, UCP2, Bcl2, Bax, and cleaved caspase-3. (b–i) Quantitative analysis of the relative protein levels after HI insult. ∗P < 0.05 vs. sham; #P < 0.05 vs. HI+vehicle; @P < 0.05 vs. HI+rh-CSF1+DMSO; n = 6 per group.
