Table 2.
The expression of four plasmids in eight different E. coli-based systems. It showed the fluorescence value of the model protein pET23a-deGFP.
| Escherichia coli | Derivation | Key feature(s) | pET23a-deGFP |
|---|---|---|---|
| BL21(DE3) | B834 | lon and ompT protease deficient | 45,496 ± 3618 |
| BL21 Star (DE3) | BL21 (DE3) | rne131 mutant, enhancing the stability of intracellular mRNA | 64,638 ± 7462 |
| Origami B (DE3) | B strain | trx /gor mutant, greatly facilitating cytoplasmic disulfide bond formation | 59,115 ± 5735 |
| Origami B (DE3) & Kan + Tet | 42,126 ± 3513 | ||
| Rosetta (DE3) | BL21 | Expressing six rare tRNAs; facilitating expression of genes that encode rare E. coli codons | 28,528 ± 4812 |
| Rosetta (DE3) & Cam | 1018 ± 65 | ||
| Rosetta-gami B (DE3) | B strain | Combining Origami B (DE3) and Rosetta (DE3) | 56,101 ± 15,027 |
| Rosetta-gami B (DE3) & Kan + Tet + Cam | 17,829 ± 5324 |