Fig. 1.

Vincristine and prednisone induce apoptosis in the first time diagnosed leukemia B cells. Cells (JM1 and SUP-B15) were seeded in a 96-well culture plate (cell density: 105/100 µl) and treated with different doses. A. Vincristine (0.1 to 4.0 µM) and B. Prednisone (0.1 to 12.0 µM) and cells were harvested at 24 and 48 h of post-treatment. Cell apoptosis was assessed by MTS assay. Each experiment was carried out in quadruplets; data represented here is the mean of each value (Mean ± S.D.).