Table 2.
Antibodies used in this study.
| Antibody | Source | Catalog No. | Host | Dilution |
|---|---|---|---|---|
| LC3B | Cell signaling | #2775S | Mouse | 34 ng/ml (WB) |
| LC3B | Sigma | #L7543 | Rabbit | 10 μg/ml (IHC) |
| Caspase 3 | Cell signaling | #9662 | Rabbit | 98 ng/ml (WB) |
| PARP-1 | Cell signaling | #9542 | Rabbit | 195.2 ng/ml (WB) |
| ATG 5 | Cell signaling | #12994 | Rabbit | 220 ng/ml (WB) |
| ATG12 | Cell signaling | #4180 | Rabbit | 40 ng/ml (WB) |
| Beclin-1 | Cell signaling | #3495 | Rabbit | 111 ng/ml (WB) |
| p-Beclin-1Ser15 | Cell signaling | # 84966 | Rabbit | 1.018 μg/ml (WB) |
| p62 | Cell signaling | #5114 | Rabbit | 18.1 ng/ml (WB) |
| p62 | Abcam | #ab56416 | Mouse | 10 μg/ml (IF) |
| SIRT-1 | Cell signaling | #9475 | Rabbit | 42 ng/ml (WB) |
| AKT | Cell signaling | #9272 | Rabbit | 34 ng/ml (WB) |
| p-AKTSer473 | Cell signaling | #9271 | Rabbit | 20 ng/ml (WB) |
| FOXO3a | Cell signaling | #9464 | Rabbit | 131 ng/ml (WB) |
| p-FOXO3aSer253 | Cell signaling | #9466 | Rabbit | 1.816 μg/ml (WB) |
| ULK-1 | Abcam | #ab167139 | Rabbit | 1 μg/ml (WB) |
| p-ULK-1Ser757 | Abcam | #ab229909 | mouse | 1.19 μg/ml (WB) |
| Wilms Tumor-1 (WT1) | Boster Biological Technology | #M00199-1 | Rabbit | 1 μg/ml (IHC) |
| Goat anti-rabbit HRP conjugate | Bio-rad | #170-6515 | Rabbit | 1:5000 (WB) |
| Goat anti-mouse HRP conjugate | Bio-rad | #170-6516 | mouse | 1:5000 (WB) |
| β-Actin | Sigma | #A5441-2ML | mouse | 1:20,000 (WB) |
| Goat anti-mouse IgG-Alexa Fluor 594 | Abcam | #ab150116 | Mouse | 2 μg/ml (IF) |