Figure 1.

Amplification of DNA (100 pg/µl, 10 pg/µl, 1 pg/µl) with different primer concentrations ranging from 200 nM to 1000 nM. The amplification was grouped tightly by the concentration of DNA. The higher the DNA concentration, the earlier the amplification started (lower Ct value). Primer concentrations had no significant differences in the amplification efficiencies with a very small change of Ct value. The three arrows indicated the DNA concentration for each group was 100 pg/µl, 10 pg/µl, and 1 pg/µl from left to right. A. pst; B. caf1; C. ypo2088; D. opgG; E. 18S rRNA