Table 4.

Optimization of primer/probe concentration and identification of limit of detection (LOD) of each gene used in the pentaplex assay

Gene	Primer concentration (nM)	Probe concentration (nM)	LOD (fg)
pst	200 nM	200 nM	1
caf1	200 nM	200 nM	50
ypo2088	200 nM	200 nM	100
opgG	600 nM	200 nM	5
18S rRNA	500 nM	200 nM	1