Fig. 5.

Differences in T cell maturation phenotype and CD62L expression between memory/balanced and naive type. Peripheral blood mononuclear cells were isolated from 15 MS patients before anti-CD20 antibody treatment was initiated (filled triangles, before depletion; n = 8/7 samples), after 1 to 5 mo (thin triangles, early depletion; n = 7/5 samples), after 6 to 8 mo (thick triangles, late depletion; n = 5/5 samples), and after 8 to 24 mo (filled triangles, at reappearance = at B cell reappearance; n = 6/4 samples). The patients were classified as memory/balanced type or naive type as described in Fig. 1. Depicted are dot plots showing the mean ± SEM. Frequency of naive cells (T_N, CD62L⁺ CD45RO⁻) gated on CD4⁺ cells (A) and on CD8⁺ cells (B), central memory cells (T_CM, CD62L⁺ CD45RO⁺) gated on CD4⁺ cells (C) and on CD8⁺ cells (D), and terminally differentiated cells (T_TD, CD62L⁻ CD45RO⁻; C) gated on CD4⁺ cells (E) and on CD8⁺ cells (F), as well as frequency of CD62L⁺ CD4⁺ T cells (G) and CD8⁺ T cells (H) (*P < 0.05; **P < 0.005; Wilcoxon matched-pairs signed rank test/paired t test; α value was corrected with the Bonferroni–Holm method; difference between the two groups [D]: unpaired t test).