Fig. 3.

Sample preparation methods tested with SHERLOCK P. falciparum assay using simulated malaria samples of live intraerythrocytic P. falciparum spiked into whole blood at 1 fM (602 parasites per microliter) concentration. (A) Detergents and heating methods assessed for SHERLOCK compatibility. (B) Combinations of chelating and reducing agents tested for optimization of chemical deactivation of nucleases and inhibitors. Asterisks indicate significant differences from untreated simulated whole blood sample assessed by Student’s two-tailed t test. Bars: mean ± SD of three technical replicates. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant.