Fig. 5.

Rev1 processivity. The Rev1 processivity assay in the presence of MgCl₂ (A) or MnCl₂ (B). Each processivity assay was repeated at least three times. (C) An active site close-up of the Rev1 product complex after a 240-min soak in MnCl₂. The nucleic acid residues are shown in yellow, Mn²⁺ in purple, and Rev1 in gray. An Fo–Fc OMIT map contoured at σ = 3.0 is shown as a green mesh. The inserted dCMP, second bound dCTP, and key residues are indicated. (D) The same structure and view as in C with Rev1 shown in a gray surface representation. (E) An active site close-up of the L325G Rev1 product complex after a 20-min soak in MnCl₂. The nucleic acid residues are shown in yellow, Mn²⁺ in purple, and Rev1 in gray. An Fo–Fc OMIT map contoured at σ = 3.0 is shown as a green mesh. The inserted dCMP, second bound dCTP, and key residues are indicated. (F) The same structure and view as in E with Rev1 shown in a gray surface representation.