Fig. 2.

Auxiliary glutamate receptor subunit dSol-1 is required for rapid PHP expression. (A) Protein structure of Neto2, Neto, Sol-1, and dSol-1 (from the indicated organisms), with the region targeted by the single-guide RNA to generate the dSol-1^bk1 and dSol-1^bk2 mutant alleles and their predicted protein products indicated below. (B) Representative mEPSP and EPSP traces in wild type (w¹¹¹⁸), dSol-1^bk1 (w;dSol-1^bk1), dSol-1^bk2 (w;dSol-1^bk2), dSol-1^bk1/Df [w;dSol-1^bk1/Df(3R)Exel7315], and dSol-1^silent (w;dSol-1^silent) at baseline and after PhTx application. While PhTx application reduces mEPSP amplitudes in all genotypes, as expected, EPSP amplitude fails to return to baseline levels in all dSol-1 mutant alleles and combinations following PhTx application due to a failure to enhance presynaptic neurotransmitter release (quantal content). (C) Quantification of mEPSP amplitude and quantal content values in the indicated genotypes following PhTx application relative to baseline (−PhTx). (D) Representative mEPSC and EPSC traces in two-electrode voltage-clamp configuration in the indicated genotypes at baseline and after PhTx application. (E) Quantification of mEPSC amplitude and quantal content TEVC values in the indicated genotypes and conditions. Error bars indicate ±SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not significant. Absolute values for normalized data are summarized in SI Appendix, Table S2.